The yeast-like fungus Aureobasidium thailandense LB01 produces a new biosurfactant using olive oil mill wastewater as an inducer

Dayana P. Meneses, Eduardo J. Gudiña, Fabiano Fernandes, Luciana R.B. Gonçalves, Lígia R. Rodrigues and Sueli Rodrigues; Microbiological Research; 204; 40–47  (2017)


Optimization of fermentation conditions for the production of curcumin by engineered Escherichia coli

Márcia R. Couto, Joana L. Rodrigues and Lígia R. Rodrigues; J. R. Soc. Interface; 14; 20170470 (2017)


Extraction and recovery processes for cynaropicrin from Cynara cardunculus L. using aqueous solutions of surface-active ionic liquids

Emanuelle L. P. de Faria, Melissa V. Gomes, Ana Filipa M. Cláudio, Carmen S. R. Freire, Armando J. D. Silvestre, Mara G. Freire; Biophysical Reviews; 10; 915–925 (2018)


Is it possible to create ternary-like aqueous biphasic systems with deep eutectic solvents?

Fabiane Oliveira Farias, Helena Passos, Álvaro S. Lima, Marcos R. Mafra and João A. P. Coutinho; ACS Sustainable Chem. Eng.; DOI: 10.1021/acssuschemeng.7b02514 (2018)


Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters

Joana L. Rodriguesa, Márcia R. Couto, Rafael G. Araújo, Kristala L.J. Prather, Leon Kluskens and Lígia R. Rodrigues; Biochemical Engineering Journal; 125; 41–49 (2017)


Deep eutectic solvents as efficient media for the extractio and recovery of cynaripicrin from Cynara cardunculus L. leaves

Emanuelle L. P. de Faria, Rafael S. do Carmo, Ana Filipa M. Cláudio, Carmen S. R. Freire, Mara G. Freire and Armando J. D. Silvestre; Int. J. Mol. Sci.; 18; 2276 (2017)


Aqueous solutions of surface-active ionic liquids remarkable alternative solvents to improve the solubility of triterpenic acids and their extraction from biomass

Emanuelle L. P. de Faria, Selesa V. Shabudin, Ana Filipa M. Claúdio, Mónica Válega, Fernando M. J. Domingues, Carmen S. R. Freire, Armando J. D. Silvestre and Mara G. Freire; ACS Sustainable Chem. Eng.; 5; 7344−7351 (2017)


Vineyard pruning waste as an alternative carbon source to produce novel biosurfactants by Lactobacillus paracasei

X. Vecino, L. Rodríguez-López, E.J. Gudiña, J.M. Cruz, A.B. Moldes and L.R. Rodrigues; Journal of Industrial and Engineering Chemistry; 55; 40–49 (2017)


Using ionic liquids to tune the performance of aqueous biphasic systems based on pluronic L-35 for the purification of Naringin and Rutin

Francisca A. e Silva, Rui M. C. Carmo, Andreia P. M. Fernandes, Mariam Kholany, João A. P. Coutinho and Sónia P. M. Ventura; ACS Sustainable Chem. Eng.; 5; 6409−6419 (2017)


Toward an understanding of the mechanisms behind the formation of liquids-liquids systems formed by two ionic liquids

Catarina M. S. S. Neves, Artur M. S. Silva, Ana M. Fernandes, João A. P. Coutinho and Mara G. Freire; J. Phys. Chem. Lett.; 8; 3015−3019 (2017)


Separation of phenolic compounds by centrifugal parition chromatography

João H. P. M. Santos, Mafalda R. Almeida, Cláudia I. R. Martins, Ana C. R. V. Dias, Mara G. Freire, João A. P. Coutinho and Sónia P. M. Ventura; Green Chemistry; DOI: 10.1039/c8gc00179k (2018)


Designer solvent ability of alcohols in aqueous biphasic systems composed of deep eutectic solvents and potassium phosphate

Fabiane Oliveira Farias, Helena Passos, Matheus Gomes Sanglard, Luciana Igarashi-Mafra, João A.P. Coutinho, Marcos R. Mafra; Separation and Purification Technology; 200; 84-93; DOI: 10.1016/j.seppur.2018.02.029 (2018)


pH Effect on the Formation of Deep-Eutectic-Solvent-Based Aqueous Two-Phase Systems

F. O. Farias; H. Passos; J. A. P. Coutinho; M. R. Mafra; Ind. Eng. Chem. Res.; 57; 16917–16924 (2018)


Cloud Point Extraction of Chlorophylls from Spinach Leaves Using Aqueous Solutions of non-Ionic Surfactants

Ana C. Leite, Ana Maria Ferreira, Eduarda Morais, Imran Khan, Mara G. Freire and João A.P. Coutinho; ACS Sustainable Chemistry and Engineering; 6; 590-599; DOI: 10.1021/acssuschemeng.7b02931  (2018)


Hydrogen bond basicity of ionic liquids and molar entropy of hydration of salts as major descriptors in the formation of aqueous biphasic systems

Helena Passos, Teresa B. V. Dinis, Ana Filipa M. Cláudio, Mara G. Freire and João A. P. Coutinho; Phys. Chem. Chem. Phys.; 20; 14234; DOI: 10.1039/c8cp01401a (2018)


New β-galactosidase producers with potential for prebiotic synthesis

Sara C. Silvério, Eugénia A. Macedo, José A. Teixeira, Lígia R. Rodrigues; Bioresource Technology; 250; 131–139 (2018)


Deep Eutectic Solvent Aqueous Solutions as Efficient Media for the Solubilization of Hardwood Xylans

Eduarda S. Morais, Patrícia V. Mendonça, Jorge F. J. Coelho, Mara G. Freire, Carmen S. R. Freire, João A. P. Coutinho and Armando J. D. Silvestre; ChemSusChem; 11; 1 – 11 (2018)


Valorization of olive tree leaves: Extraction of oleanolic acid using aqueous solutions of surface-active ionic liquids

Ana Filipa M. Cláudio, Alice Cognigni, Emanuelle L.P. de Faria, Armando J.D. Silvestre, Ronald Zirbs, Mara G. Freire, Katharina Bica; Separation and Purification Technology; 204; 30–37 (2018)


Recent developments in the functionalization of betulinic acid and its natural analogues: a route to new bioactive compounds

J. L. C. Sousa, C. S. R. Freire, A. J. D. Silvestre, A. M. S. Silva, Molecules; 24; 355 (2019)


Recombinant family 3 carbohydrate-binding module as a new additive for enhanced enzymatic saccharification of whole slurry from autohydrolyzed Eucalyptus globulus wood

Carla Oliveira, Aloia Romaní, Daniel Gomes, Joana T. Cunha, Francisco M. Gama, Lucília Domingues; Cellulose; 25; 2505–2514 (2018)


HAA1 and PRS3 overexpression boosts yeast tolerance towards acetic acid improving xylose or glucose consumption: unravelling the underlying mechanisms

Joana T. Cunha, Carlos E. Costa, Luís Ferraz, Aloia Romaní, Björn Johansson, Isabel Sá-Correia, Lucília Domingues; Applied Microbiology and Biotechnology; 102; 4589–4600 (2018)


Comparative autohydrolysis study of two mixtures of forest and marginal land resources for co-production of biofuels and value-added compounds

Rita Pontes, Aloia Romaní, Michele Michelin, Lucília Domingues, Jose Teixeira, Joao Nunes; Renewable Energy; 128; 20-29 (2018)


Production of fructo-oligosaccharides by Aspergillus ibericus and their chemical characterization

C. Nobre, E.G. Alves Filho, F.A.N. Fernandes, E.S. Brito, S. Rodrigues, J.A. Teixeira, L.R. Rodrigues; LWT – Food Science and Technology; 89; 58–64 (2018)


One-Step Co-Culture Fermentation Strategy to Produce High-Content Fructo-Oligosaccharides

C. Nobre; D. A. Goncalves;J. A. Teixeira; L. R. Rodrigues;  Carbohydr. Polym.201; 31–38 (2018)


Sodium chloride effect on the aggregation behaviour of rhamnolipids and their antifungal activity

Ana I. Rodrigues, Eduardo J. Gudiña, José A. Teixeira and Lígia R. Rodrigues; Scientific Reports; 7; 12907; DOI:10.1038/s41598-017-13424-x (2018)


Xylitol production from lignocellulosic whole slurry corn cob by engineered industrial Saccharomyces cerevisiae PE-2

S. L. Baptista , J. T. Cunha, A. Romaní, L. Domingues; Bioresour. Technol. 267:481–491 (2018)


Molecular and physiological basis of Saccharomyces cerevisiae tolerance to adverse lignocellulose-based process conditions

J. T. Cunha, A. Romaní , C. E. Costa, I. Sá-correia, L.  Domingues;  Appl Microb Biotech; 103, 1, 159–175 (2018)


Insights into the economic viability of cellulases recycling on bioethanol production from recycled paper sludge

D. G. Gomes, S. Serna-loaiza, C. A. Cardona, M. Gama, L. Domingues; Bioresour. Technol. 267; 347–355 (2018)


Determinants on an efficient cellulase recycling process for the production of bioethanol from recycled paper sludge under high solid loadings

D. Gomes, M. Gama, L. Domingues; Biotechnol. Biofuels; 11 (2018)


Recombinant family 3 carbohydrate-binding module as a new additive for enhanced enzymatic saccharification of whole slurry from autohydrolyzed Eucalyptus globulus wood

C. Oliveira, A. Romaní, D. Gomes, J. T. Cunha, Gama, L. Domingues; Cellulose; 25;4; 2505-2513 (2018)


Comparative autohydrolysis study of two mixtures of forest and marginal land resources for co-production of biofuels and value-added compounds

R. Pontes, A. Romaní, M. Michelin, L. Domingues, J. A. Teixeira, J. Nunes; Renewable Energy; 128; 20–29 (2018)


Methane production and conductive materials: a critical review

Gilberto Martins; Andreia F. Salvador; Luciana Pereira; M. Madalena Alves; Environmental Science and Technology, 52; 18, 10241-10253 (2018)


Photocatalytic degradation of diclofenac by hydroxyapatite-TiO2 composite material: identification of transformation products and assessment of toxicity

S. Murgolo; I. S. Moreira; C. Piccirillo; P. M. L. Castro; G. Ventrella; C. Cocozza; G. Mascolo; Materials; 11; 1779 (2018)


Comparative analysis of methods and models for predicting biochemical methane potential of various organic substrates

R. P. Rodrigues, A. Klepacz-Smolka, R. C. Martins, M. J. Quina; Science of the Total Environment, 649, 1599-1608 (2019)


Enzymatic production of xylooligosaccharides from Brazilian Syrah grape pomace flour: a green alternative to conventional methods for adding value to agricultural by‐ products

J. R. Costa, R. V. Tonon, L. M. F. Gottschalk, M. C. P. A. Santiago, C. Mellinguer-Silva, L. Pastrana, M. M. Pintado, L. M. C. Cabral; Journal of the Science of Food and Agriculture 99; 1250-1257 (2018)


Fermented Foods and Beverages in Human Diet and Their Influence on Gut Microbiota and Health 

N. M. Carvalho, E. M. Costa, S. Silva, L. Pimentel, T. H. Fernandes, M. E. Pintado; Fermentation – Special issue Microbial Foods: The Science of fermented foods; 4; 4; 90 (2018)


Nanoencapsulation of Polyphenols towards Dairy Beverage Incorporation

S. Silva, M. Veiga, E. M. Costa, A. L. S. Oliveira, A. R. Madureira, M. Pintado; Beveraged; 4; 3; 61 (2018)


Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation

D. A. Campos, E. R. Coscueta, N. W. Valetti, L. M. Pastrana-Castro, J. A. Teixeira, G. A. Picó; M. M. Pintado; Food Hydrocolloids; 87; 792-804 (2019)


Impact of food matrix components on nutritional and functional properties of fruit-based products

A. Oliveira, A. L. Amaro, M. Pintado; Current Opinion in Food Science; 22; 153-159 (2018)


Single-Step Production of Arabino-Xylooligosaccharides by Recombinant Bacillus Subtilis 3610 Cultivated in Brewers’ Spent Grain

C. Amorim; S. C. Silverio; S. P. Silva; E. Coelho; M. A. Coimbra; K. L. J. Prather; L. R. Rodrigues; Carbohydr. Polym., 199; 546–554 (2018)


In Vitro Digestibility and Fermentability of Fructo-Oligosaccharides Produced by Aspergillus Ibericus

C. Nobre; S. C. Sousa; S. P. Silva; A. C. Pinheiro; E. Coelho;A. A. Vicente; A. M. P. Gomes; M. A. Coimbra; J. A. Teixeira; L. R. Rodrigues; J. Funct. Foods; 46; 278–287 (2018)


Improvement of Biosurfactant Production by Wickerhamomyces Anomalus CCMA 0358 and Its Potential Application in Bioremediation

K. S. T. Souza; E. J. Gudina; R. F. Schwan; L. R. Rodrigues; D. R. Dias; J. A. Teixeira; J. Hazard. Mater.;346; 152–158 (2018)


Chemical Composition of Lipophilic Bark Extracts from Pinus Pinaster and Pinus Pinea Cultivated in Portugal

J. L. C. Sousa; P. A. B. Ramos; C. S. R. Freire; A. M. S. Silva; A. J. D. Silvestre; Appl. Sci.; 8 (2018)


Bioactivity of Glycolipopeptide Cell-Bound Biosurfactants against Skin Pathogens

X. Vecino; L. Rodriguez-Lopez; D. Ferreira; J. M. Cruz; A. B. Moldes; L. R. Rodrigues; Int. J. Biol. Macromol.; 109; 971–979 (2018)


Recent developments in the functionalization of betulinic acid and its natural analogues: A route to new bioactive compounds

Sousa, J. L. C.; Freire, C. S. R.; Silvestre, A. J. D.; Silva, A. M. S.; Molecules; 24, 2; 355 (2019)


Analysis of Process Alternatives for Energy-Efficient Bioethanol Downstream Processing

Nunes, D.S., Granjo, J.F.O., Duarte, B.P.M., Oliveira, N.M.C.; Computer Aided Chemical Engineering; 46; 391-396 (2019)


Enhanced Conversion of Xylan into Furfural using Acidic Deep Eutectic Solvents with Dual Solvent and Catalyst Behavior

Morais, E.S., Freire, M.G., Freire, C.S.R., Coutinho, J.A.P., Silvestre, A.J.D.; ChemSusChem; http://DOI: 10.1002/cssc.201902848; (2019)


High pressure extraction of bioactive diterpenes from the macroalgae: Bifurcaria bifurcata: An efficient and environmentally friendly approach

Morais, E.S., Freire, M.G., Freire, C.S.R., Coutinho, J.A.P., Silvestre, A.J.D.; RSC Adv., 2019, 9, 39893 (2019)


What a difference a methyl group makes-probing choline-urea molecular interactions through urea structure modification

Liliana P. Silva, Catarina F. Araújo, Dinis O. Abranches, Manuel Melle-Franco, Mónia A. R. Martins, Mariela M. Nolasco, Paulo J. A. Ribeiro-Claro, Simão P. Pinho, João A. P. Coutinho; Phys. Chem. Chem. Phys.; 21; 18278 (2019)


Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways

Joana T. Cunha; Pedro O. Soares; Aloia Romaní; Johan M. Thevelein; Lucília Domingues; Biotechnology for Biofuels; 12(1),20; (2019)


Impact of: In vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv. Syrah grape pomace extract

Costa, J.R.Amorim, M.Vilas-Boas, A.Tonon, R.V.Cabral, L.M.C.Pastrana, L.Pintado, MFood Funct.;10; 1856-1869; (2019)


Garden and food waste co-fermentation for biohydrogen and biomethane production in a two-step hyperthermophilic-mesophilic process

Abreu, A.A., Tavares, F., Alves, M.M., Cavaleiro, A.J., Pereira, M.A.; Bioresource Technology; 278, pp. 180-186 (2019)


Valorization of Eucalyptus nitens bark by organosolv pretreatment for the production of advanced biofuels

Romaní, A., Larramendi, A., Yáñez, R., (…), Teixeira, J.A., Domingues, L.; Industrial Crops and Products; 132, pp. 327-335 (2019)


Valorisation of bark lipophilic fractions from three Portuguese Salix species: A systematic study of the chemical composition and inhibitory activity on Escherichia coli

Ramos, P.A.B., Moreirinha, C., Santos, S.A.O., (…), Silva, A.M.S., Silvestre, A.J.D.; Industrial Crops and Products; 132, pp. 245-252 (2019)


Synthesis and characterization of chiral ionic liquids based on quinine, L-proline and L-valine for enantiomeric recognition

Sintra, T.E., Gantman, M.G., Ventura, S.P.M., (…), Wasserscheid, P., Schulz, P.S.; Journal of Molecular Liquids; 283, pp. 410-416 (2019)


Can cholinium chloride form eutectic solvents with organic chloride-based salts?

Abranches, D.O., Silva, L.P., Martins, M.A.R., (…), Pinho, S.P., Coutinho, J.A.P.; Fluid Phase Equilibria; 493, pp. 120-126 (2019)


Recovery of Syringic Acid from Industrial Food Waste with Aqueous Solutions of Ionic Liquids

De Faria, E.L.P., Ferreira, A.M., Cláudio, A.F.M., (…), Silvestre, A.J.D., Freire, M.G.; ACS Sustainable Chemistry and Engineering; 7(16), pp. 14143-14152 (2019)


Using COSMO-RS to design choline chloride pharmaceutical eutectic solvents

Abranches, D.O., Larriba, M., Silva, L.P., (…), Pinho, S.P., Coutinho, J.A.P.; Fluid Phase Equilibria; 497, pp. 71-78 (2019)


The role of charge transfer in the formation of type i deep eutectic solvent-analogous ionic liquid mixtures

Abranches, D.O., Schaeffer, N., Silva, L.P., (…), Pinho, S.P., Coutinho, J.A.P.; Molecules; 24 (20); 24203687 (2019)


Assessment of Agroforestry Residues: Their Potential within the Biorefinery Context

Gaspar, M.C., Mendes, C.V.T., Pinela, S.R., (…), Braga, M.E.M., Portugal, A.T.; ACS Sustainable Chemistry and Engineering; 7(20), pp. 17154-17165 (2019)


Intensifying ethanol production from brewer’s spent grain waste: Use of whole slurry at high solid loadings

Pinheiro, T., Coelho, E., Romaní, A., Domingues, L.; New Biotechnology; 53, pp. 1-8 (2019)


Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains

Díaz-Fernández, D., Aguiar, T.Q., Martín, V.I., (…), Revuelta, J.L., Jiménez, A.; Bioresource Technology; 293,122054 (2019)


The health-promoting potential of Salix spp. Bark polar extracts: Key insights on phenolic composition and in vitro bioactivity and biocompatibility

Ramos, P.A.B., Moreirinha, C., Silva, S., (…), Silva, A.M.S., Silvestre, A.J.D.; Antioxidants; 8(12),609 (2019)


Uncovering the potentialities of protic ionic liquids based on alkanolammonium and carboxylate ions and their aqueous solutions as non-derivatizing solvents of Kraft lignin

Dias, R.M., da Costa Lopes, A.M., Silvestre, A.J.D., Coutinho, J.A.P., da Costa, M.C.; Industrial Crops and Products; 143,111866 (2020)


Recent trends on seaweed fractionation for liquid biofuels production

del Río, P.G., Gomes-Dias, J.S., Rocha, C.M.R., (…), Garrote, G., Domingues, L.; Bioresource Technology; 299,122613 (2020)


Chromatographic separation of betulinic and oleanolic acids

Azenha, I.S., Aniceto, J.P.S., Sequeira, S.P., Mendes, A., Silva, C.M.; Separation and Purification Technology; 235,116129 (2020)


Environmentally-safe scco2 p. pinaster branches extracts: Composition and properties

Gaspar, M.C., De Sousa, H.C., Seabra, I.J., Braga, M.E.M.; Journal of CO2 Utilization; 37, pp. 74-84 (2020)


A comparison of process alternatives for energy-efficient bioethanol downstream processing

Granjo, J.F.O., Nunes, D.S., Duarte, B.P.M., Oliveira, N.M.C.; Separation and Purification Technology; 238,116414 (2020)




Book Chapter

Chapter 17: Non-extractable phenolic compounds as food ingredients in Non-extractable polyphenols and carotenoids

S. Silva, A. L. S. Oliveira, T. Ribeiro, A. R. Madureira, M. M. Pintado; 345-366 (2018)

Edited by F Saura-Calixto and j Perez-Jimenez, Royal Society of Chemistry, Croydon, UK


Chapter 34: Valorization of Residues from Fig Processing Industry by Anaerobic Digestion

D. P. Rodrigues; C. I. Alves; R. C. Martins; M. J. Quina; A. Klepacz-Smolka; M. N. Coelho Pinheiro; L. M. Castro; Wastes – Solut. Treat. Oppor. Ii; 221–226 (2018)

Selected Papers from the 4th Edition of the International Conference on Wastes: Solutions, Treatments and Opportunities, Porto, Portugal

Edited ByCandida Vilarinho, Fernando Castro, Maria de Lurdes Lopes